WESTERN BLOTTING

An established biochemical technique for detecting specific proteins in complex samples is Western Blotting (also known as immunoblotting). It can be used in a range of applications from basic research to the diagnosis of infectious diseases. This technique can determine quantity, molecular weight and post-translational modifications of proteins and can therefore be a powerful tool to monitor changes in proteins, including expression and modifications.

Existing Western Blot imaging systems can be bulky, expensive and have poor sensitivity, because they use either scanning line sensors or lens-coupled CCDs. Spectrum Logic’s has developed a western blot imager which will enable accurate laboratory testing – the Spectrum Logic CL1510 1:1 imager.  It can be ramped up inexpensively using a unique, large-area CMOS image sensor coupled to fiber optic faceplate for 1:1 imaging without a lens or scanning. This allows the Spectrum Logic CL1510 1:1 imager to capture much more of the light emitted during the assay, making it more sensitive. 1:1 imaging with a fiber optic plate also provides very sharp images and is not prone to inaccurate focusing of a lens. With an onboard computer and light-tight, waterproof bench-top enclosure, the Spectrum Logic CL1510 1:1 imager can simply be plugged into a laptop and turned on, achieving results in minutes.

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